Pavlovian Fear Conditioning Task

HED Task ID: hedtsk_pavlovian_fear_conditioning

Also known as: Fear Conditioning, Classical Conditioning, FC

A neutral CS is paired with an aversive US (shock, loud sound); conditioned responses (SCR, startle, amygdala BOLD) index fear learning.

Description

A neutral conditioned stimulus (CS; auditory tone or visual cue) is paired with an aversive unconditioned stimulus (US; mild shock, loud noise). After conditioning, the CS alone elicits conditioned fear responses including skin conductance increases, heart rate changes, freezing (in animals), and subjective fear ratings (in humans). Extinction is measured in a subsequent phase where the CS is presented without the US. The task is fundamental to understanding emotional learning and has been used extensively to study the amygdala’s role in fear acquisition and the prefrontal cortex’s role in extinction.

Inclusion test

Procedure

Participants view a neutral stimulus (CS+) paired with an aversive unconditioned stimulus (US, typically mild shock or loud noise); another stimulus (CS−) is never paired. Acquisition is followed by extinction (CS+ presented without US).

Manipulation

CS+/CS− discrimination; reinforcement schedule (partial vs. 100%); extinction timing; reinstatement/renewal context.

Measurement

Differential SCR (CS+ > CS−); startle potentiation; fMRI amygdala and vmPFC activation; fear ratings; extinction learning curve.

Variations

Variation

Description

Justification

Delay Conditioning

CS co-terminates with US; standard amygdala-dependent paradigm.

CS and US overlap; canonical associative fear learning

Trace Conditioning

Temporal gap between CS offset and US onset; hippocampus-dependent.

Trace interval between CS offset and US onset; different temporal structure

Contextual Conditioning

Context (room, background) becomes CS; hippocampus-dependent.

Context (environment) as CS; different stimulus type

Differential Conditioning (CS+/CS−)

Two CSs—one paired, one unpaired; standard design for specificity.

Two CSs with different US contingencies; adds discrimination demand

Extinction Training

CS+ presented without US after acquisition; new inhibitory learning.

CS presented without US; tests extinction learning

Extinction Recall

Testing extinction retention after delay (typically 24 hours); probes consolidation.

Test of extinguished fear after delay; different memory phase

Renewal (Context Change)

After extinction, return to acquisition context; fear returns.

Extinguished CS tested in new context; tests context-specificity of extinction

Reinstatement

Unsignaled US presentation after extinction; assesses fear recovery.

Unsignaled US presentations revive extinguished fear; different recovery procedure

Reconsolidation Paradigm

Brief CS re-exposure during reconsolidation window followed by extinction; targets memory updating.

Reactivation + interference during reconsolidation; different intervention timing

Generalization Gradient

Testing stimuli that vary parametrically from CS+; maps fear generalization.

CS-similar stimuli tested; measures perceptual generalization

Instructed vs. Uninstructed

Explicit information about CS-US contingency vs. learning through experience only.

Verbal instruction about CS-US relationship; tests instructed vs. experiential fear

Compound CS Conditioning

Multiple cues paired with US; tests configural vs. elemental learning.

CS compound with multiple elements; different stimulus structure

Cognitive processes

This task engages the following cognitive processes:

Key references

  • {‘authors’: ‘LeDoux, J. E.’, ‘year’: 2000, ‘title’: ‘Emotion Circuits in the Brain’, ‘venue’: ‘Annual Review of Neuroscience’, ‘venue_type’: ‘journal’, ‘journal’: ‘Annual Review of Neuroscience’, ‘volume’: ‘23’, ‘issue’: ‘1’, ‘pages’: ‘155-184’, ‘doi’: ‘10.1146/annurev.neuro.23.1.155’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘LeDoux, J. E. (2000). Emotion circuits in the brain. Annual Review of Neuroscience, 23, 155-184.’, ‘url’: ‘https://doi.org/10.1146/annurev.neuro.23.1.155’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘Phelps, E. A., Delgado, M. R., Nearing, K. I., & LeDoux, J. E.’, ‘year’: 2004, ‘title’: ‘Extinction Learning in Humans’, ‘venue’: ‘Neuron’, ‘venue_type’: ‘journal’, ‘journal’: ‘Neuron’, ‘volume’: ‘43’, ‘issue’: ‘6’, ‘pages’: ‘897-905’, ‘doi’: ‘10.1016/j.neuron.2004.08.042’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘Phelps, E. A., Delgado, M. R., Nearing, K. I., & LeDoux, J. E. (2004). Extinction learning in humans: Role of the amygdala and vmPFC. Neuron, 43(6), 897-905.’, ‘url’: ‘https://doi.org/10.1016/j.neuron.2004.08.042’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘Mueller, D., Porter, J. T., & Quirk, G. J.’, ‘year’: 2008, ‘title’: ‘Noradrenergic Signaling in Infralimbic Cortex Increases Cell Excitability and Strengthens Memory for Fear Extinction’, ‘venue’: ‘The Journal of Neuroscience’, ‘venue_type’: ‘journal’, ‘journal’: ‘The Journal of Neuroscience’, ‘volume’: ‘28’, ‘issue’: ‘2’, ‘pages’: ‘369-375’, ‘doi’: ‘10.1523/jneurosci.3248-07.2008’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘Quirk, G. J., & Mueller, D. (2008). Neural mechanisms of extinction learning and retrieval. Neuropsychopharmacology, 33(1), 56-72.’, ‘url’: ‘https://doi.org/10.1523/jneurosci.3248-07.2008’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

Recent references

  • {‘authors’: ‘Fullana, M. A., Harrison, B. J., Soriano-Mas, C., et al.’, ‘year’: 2016, ‘title’: ‘Neural signatures of human fear conditioning: An updated and extended meta-analysis of fMRI studies’, ‘venue’: ‘Molecular Psychiatry’, ‘venue_type’: ‘journal’, ‘journal’: ‘Molecular Psychiatry’, ‘volume’: ‘21’, ‘issue’: ‘4’, ‘pages’: ‘500–508’, ‘doi’: None, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘Fullana, M. A., Harrison, B. J., Soriano-Mas, C., et al. (2016). Neural signatures of human fear conditioning: An updated and extended meta-analysis of fMRI studies. Molecular Psychiatry, 21(4), 500–508.’, ‘url’: None, ‘source’: ‘unresolved’, ‘confidence’: ‘none’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘Lonsdorf, T. B., Menz, M. M., Andreatta, M., Fullana, M. A., Golkar, A., Haaker, J., Heitland, I., Hermann, A., Kuhn, M., Kruse, O., Meir Drexler, S., Meulders, A., Nees, F., Pittig, A., Richter, J., Römer, S., Shiban, Y., Schmitz, A., Straube, B., Vervliet, B., Wendt, J., Baas, J. M., & Merz, C. J.’, ‘year’: 2017, ‘title’: ‘Don’t fear ‘fear conditioning’: Methodological considerations for the design and analysis of studies on human fear acquisition, extinction, and return of fear’, ‘venue’: ‘Neuroscience & Biobehavioral Reviews’, ‘venue_type’: ‘journal’, ‘journal’: ‘Neuroscience & Biobehavioral Reviews’, ‘volume’: ‘77’, ‘issue’: None, ‘pages’: ‘247-285’, ‘doi’: ‘10.1016/j.neubiorev.2017.02.026’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: “Lonsdorf, T. B., Menz, M. M., Andreatta, M., et al. (2017). Don’t fear ‘fear conditioning’: Methodological considerations for the design and analysis of studies on human fear acquisition, extinction, and return of fear. Neuroscience & Biobehavioral Reviews, 77, 247–285.”, ‘url’: ‘https://doi.org/10.1016/j.neubiorev.2017.02.026’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘Duits, P., Cath, D. C., Lissek, S., Hox, J. J., Hamm, A. O., Engelhard, I. M., van den Hout, M. A., & Baas, J. M. P.’, ‘year’: 2015, ‘title’: ‘UPDATED META-ANALYSIS OF CLASSICAL FEAR CONDITIONING IN THE ANXIETY DISORDERS’, ‘venue’: ‘Depression and Anxiety’, ‘venue_type’: ‘journal’, ‘journal’: ‘Depression and Anxiety’, ‘volume’: ‘32’, ‘issue’: ‘4’, ‘pages’: ‘239-253’, ‘doi’: ‘10.1002/da.22353’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘Duits, P., Cath, D. C., Lissek, S., et al. (2015). Updated meta-analysis of classical fear conditioning in the anxiety disorders. Depression and Anxiety, 32(4), 239–253.’, ‘url’: ‘https://doi.org/10.1002/da.22353’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘Radua, J., Savage, H. S., Vilajosana, E., et al.’, ‘year’: 2025, ‘title’: ‘Reviewer #3 (Public review): The neural correlates of novelty and variability in human decision-making under an active inference framework’, ‘venue’: ‘Nature Communications’, ‘venue_type’: ‘other’, ‘journal’: None, ‘volume’: ‘16’, ‘issue’: ‘1’, ‘pages’: None, ‘doi’: ‘10.7554/elife.92892.4.sa2’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘Radua, J., Savage, H. S., Vilajosana, E., et al. (2025). Neural correlates of human fear conditioning and sources of variability in 2199 individuals. Nature Communications, 16(1), 7869.’, ‘url’: ‘https://doi.org/10.7554/elife.92892.4.sa2’, ‘source’: ‘crossref’, ‘confidence’: ‘high’, ‘verified_on’: ‘2026-04-20’}

  • {‘authors’: ‘López-Blanco, A., et al.’, ‘year’: 2025, ‘title’: ‘Pharmacological enhancement of fear extinction’, ‘venue’: ‘Trends in Cognitive Sciences’, ‘venue_type’: ‘journal’, ‘journal’: ‘Trends in Cognitive Sciences’, ‘volume’: None, ‘issue’: None, ‘pages’: None, ‘doi’: ‘10.1016/j.tics.2025.xxx’, ‘openalex_id’: None, ‘pmid’: None, ‘citation_string’: ‘López-Blanco, A., et al. (2025). Pharmacological enhancement of fear extinction. Trends in Cognitive Sciences, 29(2). doi:10.1016/j.tics.2025.xxx’, ‘url’: None, ‘source’: ‘needs_review’, ‘confidence’: ‘low’, ‘verified_on’: ‘2026-04-20’}